Histidine Decarboxylaseless Mutants of Lactobacillus 30a: Isolation and Growth Properties
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چکیده
منابع مشابه
Nutritional Requirements of Lactobacillus 30a for Growth and Histidine Decarboxylase Production.
Guirard, Beverly M. (University of California, Berkeley), and Esmond E. Snell. Nutritional requirements of Lactobacillus 30a for growth and histidine decarboxylase production. J. Bacteriol. 87:370-376. 1964.-The nutritional requirements of Lactobacillus 30a include each of the naturally occurring amino acids, several B vitamins, ascorbic acid, glucose, acetate, and oleate. The nutritional requi...
متن کاملStructure and cooperativity of a T-state mutant of histidine decarboxylase from Lactobacillus 30a.
Histidine decarboxylase (HDC) from Lactobacillus 30a converts histidine to histamine, a process that enables the bacteria to maintain the optimum pH range for cell growth. HDC is regulated by pH; it is active at low pH and inactive at neutral to alkaline pH. The X-ray structure of HDC at pH 8 revealed that a helix was disordered, resulting in the disruption of the substrate-binding site. The HD...
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A series of mutant strains of Lactobacillus fermenti resistant to the growth inhibitory properties of amprolium were isolated by serial transfers of the original strain in media which contained increasing concentrations of amprolium. Using three different resistance levels of strains (resistant strain A, B and C), the mechanism of amprolium resistance was investigated. Through the present studi...
متن کاملpH-induced structural changes regulate histidine decarboxylase activity in Lactobacillus 30a.
Histidine decarboxylase (HDC) from Lactobacillus 30a produces histamine that is essential to counter waste acids, and to optimize cell growth. The HDC trimer is active at low pH and inactive at neutral to alkaline pH. We have solved the X-ray structure of HDC at pH 8 and revealed the novel mechanism of pH regulation. At high pH helix B is unwound, destroying the substrate binding pocket. At aci...
متن کاملHistidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties.
At least four genetically distinct classes of mutations give rise to triazolealanine resistance in SaZmoneZZa typhimurium. Each class studied affects regulation of the histidine operon. Mutants of one class, GO, are located at one end of the histidine operon. This class may be similar to operator constitutive mutants studied in other systems. At least two genetic sites are present in this regio...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1972
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.112.1.624-626.1972